Quantitative Polymerase Chain Reaction qPCR Analysis
Quantitative Polymerase Chain Reaction qPCR is a laboratory technique used to detect a specific DNA sequence or sequences. Quantitative Polymerase Chain Reaction qPCR is common in both scientific research and diagnostics for detecting the presence of microorganisms.
One application for qPCR is the ability identify multiple pathogens at once from a variety of different specimens to determine the cause of infections. Having qPCR laboratory technology for analysis or patient samples allows doctors to quickly and more accurately choose a more specialized and specific medical therapy or pharmaceutical medicine for their patients. This may include helping them decide on which antibiotics or antiviral drugs to administer to their patients. As the application and availability of qPCR analysis expand, testing from nasal, throat, and sputum samples will have a faster turnaround time. qPCR analysis also allows laboratory analysis to be less expensive as samples can be tested for multiple bacteria or viruses at once.
While qPCR has many benefits for usage in clinical diagnostics and healthcare, qPCR also benefits the environment. Quantitative Polymerase Chain Reaction qPCR analysis can detect large numbers of genes from pathogens without having to first culture the organism. Environmental water analysis using qPCR technology allows much more effective testing to ensure safe drinking water. Previously, water testing for waterborne pathogens had to be performed one at a time. With the advancements of qPCR, multiple pathogens can be tested at once. This allows pathogens to be identified very quickly. Quickly identifying pathogens allows for an effective treatment to be determined during water pathogen outbreaks.
qPCR Test Requests Received by Contract Laboratory
- Clinical Research Organization associated with digestive health needs GLP compliant laboratory for multiple urine, faecal, blood, and gene testing Gene qPCR and metagenomics: IL-1 IL-6 IL-8 IL-10 IL-12p70 TNF and Myeloperoxidase Urine samples for Lactulose, Rhamnose, Uric acid, Vitamin C And Blood samples for Calprotectin, Uric acid, Vitamin C, Bacterial 16S rRNA, Faecal samples for Calprotectin, Gene qPCR and metagenomics, Occult bleeding, B. Breve
- A food technology company needs a Genetics Laboratory for DNA purification and QPCR analysis testing: Samples to be tested against 10 different primer/probe sets.
- LONG-TERM TESTING Ireland bioanalytical contract laboratory needed for test method transfer validation and lot release viral testing on an API for pharmaceutical use. The viral testing is both qPCR and infectivity assay. Validated test methods exist.
- Virology Laboratory needed for air testing: viral analysis per Viral indicator (Pepper Mild Mottle Virus ? PMMV) Nucleic acid extraction of PC filter followed by qPCR Genomic copy/m3 None; indicator organism Kitajima et al., 2014 Adenovirus Nucleic acid extraction of PC filter followed by qPCR Based on LD50 data Schultz et al., 2011 Norovirus Nucleic acid extraction of PC filter followed by qPCR Based on LD50 data Schultz et al., 2011
- USA Bioanalytical Laboratory needed for PCR Analysis: qPCR measurement of telomere length using the Cawthon method. We will have experimental and control samples in triplicate and would like the cost/sample (triplicate with internal control).
- Maryland EPA Environmental Laboratory needed for testing of two samples
- CLIA Bioanalytical Laboratory needed for testing for Fasciolopsis genus of parasites, specific species Fasciolopsis buskii. As well as a full comprehensive qPCR testing of the Trematoda class of parasites.
- East Coast USA Bioanalytical Laboratory needed for testing on liver samples from small animals to assess pDNA delivery to cells
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